Eosin Y (CI 45380)
Prod.-No .: A0822
Quantity
Order-No.
100 g A0822.0100
25 g A0822,0025
Synonym :
Eosin Yellowish, tetrabromofluorescein disodium salt:
C20H6Br4 Na2O5
M :691.88 g / mol
CAS-No. :17372-87-1
HS-No :32041200
EC-No. :2414096
Storage :RT
LGK :10 - 13
R :36
S :22-26:irritant
WGK :1
Transition interval :
pH 0.0 - 3.0 (yellow - green fluorescence)
Specification :
Assay (photometr.) :
min 90%λ max :
514 - 520 nm
The loss is drying :max 10%
Literature
(1) Selsted, ME & Becker III, HW (1986) Anal. Biochem 155, 270-274
Eosin Y: A Reversible Stain for Detecting Electrophoretically Resolved Protein.
Comment
The rapid staining of proteins in low-pH, urea-containing polyacrylamide gels with eosin Y reveal similar
staining intensities to those seen with Coomassie® R-250. High recovery of total protein as well
enzymatic activity were achieved (1).
Protocol : Stir the gel for 30 seconds in 0.25% Eosin Y in 0.1 N NaOH. Rinse the gel for 10 seconds with
distilled water. The eosin-stained proteins appeared as red-orange bands on a pale orange background.
Backhround staining can be reduced by extending the destaining period to 30 minutes. Much longer
Destaining in water result ed in a loss of band intensity. Staining intensity could be stabilized indefinitely by
storing the gel in 5% acetic acid after 15 - 30 minutes of destaining in water
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